RxRx

RxRx: Datasets released from Recursion’s automated cellular imaging and deep learning platform, to enable new machine learning tools and discover new biology.

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NeurIPS 2019 competition
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CellSignal: Disentangling biological signal from experimental noise in cellular images.

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To date, datasets have been released for cells treated with a broad range of perturbations, including siRNA and CRISPR/Cas9 genetic knockdown/knockout, small molecules at multiple concentrations, SARS-CoV-2 virus, immune-focused proteins, and a model of COVID-19 cytokine storm.

Release Date

June 2019

August 2020

April 2020

August 2020

January 2023

Cell Types

HUVEC
RPE
U2OS
HepG2

HUVEC

HRCE
Vero

HUVEC

Stains (Channels)

Hoechst
ConA
Phalloidin
Syto14
MitoTracker
WGA

Hoechst
ConA
Phalloidin
Syto14
WGA

Hoechst
ConA
Phalloidin
Syto14
MitoTracker
WGA

Plate Density

384-well

1536-well

Imaging Sites per Well

Perturbations Evaluated

1,138 siRNAs

434 soluble factors at 6 concentrations

1,672 small molecules at 6+ concentrations
Three viral conditions (active virus, irradiated, mock)

1,856 small molecules at 4-6 concentrations in three COVID-19-associated cytokine storm conditions (severe storm, healthy, and no cytokines)

17,063 CRISPR/Cas9-mediated gene knockouts
1,674 compounds at 8 concentrations each

Total Number of Images

125,510

131,953

305,520

70,384

~2.2M

Image Dimension

512x512x6

1024x1024x6

1024x1024x5

2048x2048x6

Compressed Dataset Size

~46GB

~185GB

~450GB

~409GB

~83,100GB

License

CC-BY-NC-SA

CC-BY

CC-BY-NC-SA